trim55 antibody (Thermo Fisher)
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90
Structured Review
Thermo Fisher
trim55 antibody
Trim55 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim55+antibody/pmc11494394-52-39-41?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
Trim55 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim55+antibody/pmc11494394-52-39-41?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
trim55 antibody - by Bioz Stars,
2026-07
90/100 stars
Images
1) Product Images from "TRIM55 Aggravates Cardiomyocyte Apoptosis After Myocardial Infarction via Modulation of the Nrf2/HO-1 Pathway"
Article Title: TRIM55 Aggravates Cardiomyocyte Apoptosis After Myocardial Infarction via Modulation of the Nrf2/HO-1 Pathway
Journal: JACC: Basic to Translational Science
doi: 10.1016/j.jacbts.2024.05.006
Figure Legend Snippet: Trim55 Expression Was Significantly Increased After MI (A, B) Quantification and representative images of Western blot analyses showing Trim55 protein expression in the border zone of myocardium at 3, 7, and 28 days after MI (n = 3). (C) Quantification of Trim55 mRNA levels in the border zone of myocardium at 3, 7, and 28 days after MI (n = 3). (D, E) Quantification and representative images of Trim55 immunohistochemistry in the border zone of myocardium at 3 and 28 days after MI (n = 3; scale bar: 50 μm). (F, G) Quantification and representative images of Western blot analyses showing Trim55 protein expression in NRCM cells after 400 μmol/L stimulation at 24 hours (n = 3). (H) Quantification of Trim55 mRNA levels in NRCM cells after CoCl 2 stimulation (400 μmol/L; 24 hours; n = 3). All data are expressed as mean ± SEM. Data in B, C, E, G, and H were analyzed using Student’s t -test. ∗ P < 0.05, ∗∗ P < 0.01 vs the control or sham group. CoCl 2 = cobalt chloride; CON = control; GADPH = glyceraldehyde-3-phosphate dehydrogenase; IHC = immunohistochemistry; MI = myocardial infarction; mRNA = messenger RNA; NRCM = neonatal rat cardiomyocyte; Trim55 = tripartite motif-containing 55.
Techniques Used: Expressing, Western Blot, Immunohistochemistry, Control
Figure 1 . " title="Trim55 Knockout Alleviated Myocardial Injury and Cardiomyocyte Apoptosis After ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Trim55 Knockout Alleviated Myocardial Injury and Cardiomyocyte Apoptosis After MI (A) Echocardiographic analysis of the LVEF and FS at 28 days after MI in the C57-sham (n = 10), Trim55 –/– -sham (n = 10), C57-MI (n = 10), and Trim55 –/– -MI (n = 10) groups. (B) Quantitative analysis of the ratio of heart weight to body weight at 28 days after MI in the C57-sham (n = 10), Trim55 –/– -sham (n = 10), C57-MI (n = 8), and Trim55 –/– -MI (n = 10) groups. (C, D) Representative HE and Masson staining pictures and quantification of the infarction area at 28 days after MI in the C57-sham, Trim55 –/– -sham, C57-MI, and Trim55 –/– -MI groups (n = 3). (E, F) Quantification and representative images of Western blot analyses showing the Trim55, collagen I, and TGF-β protein expression in the C57-sham, Trim55 –/– -sham, C57-MI, and Trim55 –/– -MI groups (n = 3). (G, H) Quantification and representative images of Western blot analyses showing the Trim55, Bax, Bcl2, cleaved caspase-3, and cleaved caspase-8 protein expression in the C57-sham (n = 3), Trim55 –/– -sham (n = 3), C57-MI (n = 4), and Trim55 –/– -MI (n = 3) groups. (I, J) Representative TUNEL staining images and quantification of apoptotic positive cells at 3 days after MI in the C57-sham (n = 3), Trim55 –/– -sham (n = 3), C57-MI (n = 3), and Trim55 –/– -MI (n = 4) groups. All data in A, B, D, F, H, and J are expressed as mean ± SEM. Data in A, B, D, F, H, and J were analyzed using 2-way analysis of variance followed by the Bonferroni post hoc test. ∗∗ P < 0.01 vs C57-sham; † P < 0.05 vs Trim55 –/– -sham; ‡ P < 0.05 vs C57-MI. Bax = BCL2 associated X apoptosis regulator; Bcl-2 = BCL2 apoptosis regulator; BW = body weight; FS = fraction shortening; HE = hematoxylin and eosin; HW = heart weight; LVEF = left ventricular ejection fraction; TGF = transforming growth factor; TUNEL = terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling; other abbreviations as in
Techniques Used: Knock-Out, Staining, Western Blot, Expressing, TUNEL Assay, End Labeling
Figures 1 and . " title="Trim55 Overexpression Promoted Cardiomyocyte Apoptosis Induced by Hypoxia (A, ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Trim55 Overexpression Promoted Cardiomyocyte Apoptosis Induced by Hypoxia (A, B) Quantification and representative images of Western blots showing the expression of apoptosis-related proteins after Trim55 overexpression (n = 3). (C, D) Effects of Trim55 overexpression and CoCl treatment on the expression of apoptosis-related proteins by Western blot (n = 3). (E, F) Effects of Trim55 overexpression and CoCl treatment on cardiomyocyte apoptosis by flow cytometry analysis (n = 4). (G, H) Effects of Trim55 overexpression and CoCl treatment on cardiomyocyte apoptosis by TUNEL staining (n = 3). CoCl : 400 μmol/L, 24 hours. All data in B, D, F, and H are expressed as mean ± SEM. Data in B were analyzed using an unpaired Student’s t -test. Data in D, F, and H were analyzed using 2-way analysis of variance followed by the Bonferroni post hoc test. ∗ P < 0.05, ∗∗ P < 0.01 vs adcon; † P < 0.05 vs adTrim55; ‡ P < 0.05 vs adcon + CoCl . adcon = adenovirus control; adTrim55 = Trim55-overexpressing adenovirus; DAPI = 4′,6-diamidino-2-phenylindole; ns = not significant; other abbreviations as in
Techniques Used: Over Expression, Western Blot, Expressing, Flow Cytometry, TUNEL Assay, Staining, Control
Figure 1 , Figure Legend Snippet: Trim55 Knockdown Alleviated Cardiomyocyte Apoptosis Induced by Hypoxia (A, B) Quantification and representative images of Western blot analyses showing the expression of apoptosis-related proteins after Trim55 knockdown (n = 3). (C, D) Effects of Trim55 knockdown and CoCl 2 treatment on the expression of apoptosis-related proteins by Western blot (n = 3). (E, F) Effects of Trim55 knockout and CoCl 2 treatment on cardiomyocyte apoptosis by flow cytometry analysis (n = 4). (G, H) Effects of Trim55 knockdown and CoCl 2 treatment on cardiomyocyte apoptosis by TUNEL staining (n = 3). All data in B, D, F, and H are expressed as mean ± SEM. Data in B were analyzed using an unpaired Student’s t -test. Data in D, F, and H were analyzed using 2-way analysis of variance, followed by the Bonferroni post hoc test. ∗ P < 0.05, ∗∗ P < 0.01 vs si-nc; ‡ P < 0.05 vs si-Trim55; † P < 0.05 vs si-nc + CoCl 2 . si-nc = siRNA-normal control; si-Trim55 = Trim55-interfering RNA; other abbreviations as in
Techniques Used: Knockdown, Western Blot, Expressing, Knock-Out, Flow Cytometry, TUNEL Assay, Staining, Control
Figure 1 , Figure Legend Snippet: Trim55 Promoted Oxidative Stress by Inhibiting the Nrf2/HO-1 Pathway in Cardiomyocytes (A) Quantification and representative images of Western blot analyses showing the expression of antioxidant proteins (P62, Nrf2, HO-1) after overexpression of Trim55 in cardiomyocytes (n = 3). (B) Quantification and representative images of Western blot analyses showing the expression of antioxidant proteins (P62, Nrf2, HO-1) after Trim55 knockdown (n = 3). (C) Effects of Trim55 overexpression on the intracellular ROS levels by flow cytometry (n = 5). (D) Effects of Trim55 silencing on the intracellular ROS levels by flow cytometry (n = 5). (E) Quantification and representative images of CellROX staining after Trim55 overexpression (n = 3). (F) Quantification and representative images of CellROX staining after Trim55 knockdown (n = 3). All data in A to F are expressed as mean ±SEM. Data in A to F were analyzed by Student’s t -test. ∗ P < 0.05, ∗∗ P < 0.01 vs adcon or si-nc. APC = allophycocyanin; HO-1 = heme oxygenase 1; ISO = isotype control; Nrf2 = nuclear factor, erythroid derived 2; ROS = reactive oxygen species; other abbreviations as in
Techniques Used: Western Blot, Expressing, Over Expression, Knockdown, Flow Cytometry, Staining, Control, Derivative Assay
Figure 1 , Figure Legend Snippet: Trim55 Promoted Cardiomyocyte Apoptosis Through Inhibiting the Nrf2/HO-1 Signaling Pathway (A) Effects of Trim55 overexpression and HO-1 overexpression on the expression of Trim55, HO-1, cleaved caspase-3, and cleaved caspase-8 by Western blot (n = 3). (B) Effects of Trim55 overexpression and HO-1 overexpression on cardiomyocyte apoptosis by flow cytometry (n = 4). (C) Effects of Trim55 overexpression and HO-1 overexpression on cardiomyocyte apoptosis by TUNEL staining (n = 3). (D) Effects of Trim55 knockdown and HO-1 knockdown on the expressions of cleaved caspase-3 and cleaved caspase-8 by Western blot (n = 3). (E) Effects of Trim55 knockdown and HO-1 knockdown on cardiomyocyte apoptosis by flow cytometry (n = 4). (F) Effects of Trim55 knockdown and HO-1 knockdown on cardiomyocyte apoptosis by TUNEL staining (n = 3). All data in A to F are expressed as mean ± SEM. Data in A to F were analyzed by 2-way analysis of variance followed by the Bonferroni post hoc test. ∗ P < 0.05, ∗∗ P < 0.01 vs adcon or si-nc; † P < 0.05 vs adTrim55 or si-nc + si HO-1; ‡ P < 0.05 vs adcon + pcDNA3.1 HO-1 or si-Trim55. Abbreviations as in
Techniques Used: Over Expression, Expressing, Western Blot, Flow Cytometry, TUNEL Assay, Staining, Knockdown
Figure 1 , Figure Legend Snippet: Foxo3 Was a Direct Upstream Transcription Factor of Trim55 (A, B) Quantification and representative images of Western blots showing the expression of Foxo3, Gata3, Hoxa5, and Stat3 after CoCl 2 hypoxia in NRCMs (n = 3). ∗ P < 0.05 vs CON. (C, D) Foxo3 expression in the myocardium post-MI. (n = 3). ∗ P < 0.05, ∗∗ P < 0.01 vs sham (E, F) Quantification and representative images of Western blot analyses showing the expression of Trim55 after Foxo3 overexpression in NRCMs (n = 3). ∗∗ P < 0.01 vs CON. (G) Quantification of Trim55 mRNA levels in NRCMs after Foxo3 overexpression (n = 3). ∗∗ P < 0.01 vs CON. (H, I) Quantification and representative images of Western blot analyses showing the expression of Trim55 after knockdown in NRCMs (n = 3). † P < 0.05 vs si-nc. (J) Quantification of Trim55 mRNA levels in NRCMs after Foxo3 knockdown (n = 3). † P < 0.05 vs si-nc. (K) Possible binding sites of Foxo3 and Trim55 were predicted using the JASPAR website. (L) Relative luciferase activity in HEK293T cells with cotransfection with Foxo3-overexpressing plasmid and with WT-Trim55 plasmid or Mut-Trim55 plasmid. ∗∗ P < 0.01 vs NC Foxo3 + WT-Trim55; ‡ P < 0.05 vs NC Foxo3 + Mut-Trim55 (n = 12). (M) The effect of Trim55 on cardiomyocyte apoptosis after MI (by Figdraw). All data in B, D, F, G, H, and J are expressed as mean ± SEM. Data in B, D, F, G, and H were analyzed using Student’s t -test. Data in J were analyzed using 2-way analysis of variance, followed by the Bonferroni post hoc test. Foxo3 = forkhead box transcription factor 3; Mut = mutated; NC = normal control; WT = wild type; other abbreviations as in
Techniques Used: Western Blot, Expressing, Over Expression, Knockdown, Binding Assay, Luciferase, Activity Assay, Cotransfection, Plasmid Preparation, Control
